Episode 25

"Goodbye Once Again" is a bonus episode that is included in the 9th DVD/BluRay volume of Kill la Kill, which was released on September 3, 2014. On June 7, 2014, Shigeto Koyama and Hiromi Wakabayashi confirmed during a Q/A session at AnimeNEXT 2014 that the theme of the OVA will be "graduation".

Plot
Two weeks after Ragyō's defeat, Honnōji Academy has served its purpose and is to be decommissioned within a month. Ryūko is at its ruins with Mako and the Elite Four watching over a friendly match between Satsuki and Sanageyama. He strikes repeatedly, but Satsuki vanishes and breaks his sword. He then is questioned by the other Elites and says Satsuki's previous resolve is gone, and as such he couldn't bring himself to strike her. Below at the town, the citizens are packing up to leave, including the Mankanshoku Family. Mataro says goodbye to his gang, advising them to enroll in elementary once they move and Sukuyo gives them "goodbye croquettes". His father reminds him they're moving as well, so he then goes to help packing by picking up their house and putting it into their truck. The camera then pins towards Honnōji Academy where the "First Graduation Ceremony" was being held.

Ryūko is sitting by herself against a wall, while Mako comes flying right at her in typical manner. Ryūko then grabs Mako and Mako tells her what school they're transferring to, "Rinne High School" in Kanagawa. Mikisugi appears near the duo in his teacher garb, saying that History teacher Mikisugi was invited. He then asks Ryūko how is she dealing with regular clothes since he was the one to arrange her and Senketsu to meet; reminding her that there are still Life Fibers in her. Mako jumps in and reminds them that Senketsu is alive within Ryūko, and she will always be wearing him. Aikurō says it's a relief and tells the futures of the Elite Four members: Gamagōri will be working at a relatives iron works, Nonon will inherit the Jakuzure Corporations, Hōka will pick an IT graduation, Iori will continue his study of Life Fibers as they move into college, and Uzu will work at home with his family while he continues kendo. He then says Satsuki is the only one left and Ryūko reply's that "she's trying to find a way to put down her sword. After spending her whole life sharpening its edge."

(More to come)

Characters

 * Ryūko Matoi
 * Satsuki Kiryūin
 * Mako Mankanshoku
 * Senketsu
 * Ragyō Kiryūin
 * Ira Gamagōri
 * Hōka Inumuta
 * Uzu Sanageyama
 * Nonon Jakuzure
 * Shirō Iori
 * Rei Hōōmaru
 * Aikurō Mikisugi
 * Tsumugu Kinagase
 * Mankanshoku Family

Trivia

 * The neon green color palettes used for most of the ghosts and weapons are based on the original concept color scheme for Senketsu.